Coding
sfGFP

Part:BBa_K3773003:Design

Designed by: Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-14)


Codon-optimized sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence completely obtained from Ceroni et al.

sfGFP was chosen because it has robust folding, resistance to denaturants, and increased ability to tolerate circular permutation, while wild-type GFP does not fold correctly in E. coli1.

Source

Ceroni, F., Algar, R., Stan, G., & Ellis, T. (2015). Quantifying cellular capacity identifies gene expression designs with reduced burden. Nature Methods, 12(5):415-418. Doi: 10.1038/nmeth.3339

References

1Pédelacq, J. D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology, 24(1), 79-88.