Coding
sfGFP
Part:BBa_K3773003:Design
Designed by: Justin Berg Group: iGEM21_William_and_Mary (2021-10-14)
Codon-optimized sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 148
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence completely obtained from Ceroni et al.
sfGFP was chosen because it has robust folding, resistance to denaturants, and increased ability to tolerate circular permutation, while wild-type GFP does not fold correctly in E. coli1.
Source
Ceroni, F., Algar, R., Stan, G., & Ellis, T. (2015). Quantifying cellular capacity identifies gene expression designs with reduced burden. Nature Methods, 12(5):415-418. Doi: 10.1038/nmeth.3339
References
1Pédelacq, J. D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology, 24(1), 79-88.